DeepAutoFocus

This is a MicroManager hardware autofocus plugin, used in Whitley et al bioRxiv 2020, developed between the Henriques and Holden laboratories. This plugin will perform microscopy autofocus while imaging a sample with visible fluorescence light, using the cross correlated signal from infrared brightfield microscopy running independently on a second camera, based on the principle described in McGorty et al, Optical Nanoscopy 2013.

🔗Source-Code Repository

Main publication:

Other publications using resource:

FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division Kevin D Whitley, Calum Jukes, Nicholas Tregidgo, Eleni Karinou, *Pedro Almada*, Yann Cesbron, *Ricardo Henriques*, Cees Dekker, Séamus Holden Published in Nature communications, April 2021 (see publication or preprint) Research themes: Microbiology, New Methods, Software, Hardware Type: Paper