Host receptor engagements is one of the first steps during HIV entry. There is still a lack of explicit knowledge on the precise stoichiometry of the virus Env-receptor interaction or of how flexible this might be for a successful fusion event, especially from the perspective of predominant viral target cells (CD4+ T cells). A better understanding of PM receptor distributions, and how this is modulated by the virus, is still required. I am aiming at characterising this dynamic process using Super-Resolution Microscopy on a single-cell level. We have successfully validated a pipeline to quantitatively analyse the topology of membrane receptors, CD4 and CCR5. Our next step is to investigate further what receptor topology makes a fusion-efficient by monitoring viral entry in living CD4+ T cells.
Research themes with our lab
Publications with our group
| Between life and death: strategies to reduce phototoxicity in super-resolution microscopy |
Kalina L Tosheva, Yue Yuan, Pedro Matos Pereira, Siân Culley, Ricardo Henriques
Published in Journal of Physics D: Applied Physics, January 2020 (see publication)