Siân Culley
Study of extremophiles and development of optical super-resolution microscopy
Started January, 2014; ended September, 2021
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My research centres on developing novel analytical approaches for fluorescence microscopy. I helped develop the Super-Resolution Radial Fluctuations (SRRF) algorithm, which extracts super-resolution information from conventional fluorescence microscopy time-series, and allows for live-cell super-resolution imaging in physiological conditions without specialised fluorophores. I also developed the Super-Resolution Quantitative Image Rating and Reporting of Error Locations (SQUIRREL) algorithm, which is a method for assessing the quality of super-resolution microscopy images. I also develop analytical methods for microbiology, including the creation of image analysis pipelines for characterising nuclear envelope architecture during mitosis in S. pombe (in collaboration with Gautam Dey, LMCB) and characterising cell division in thermophilic Archaea (in collaboration with Uwe Schmidt, MPI-CBG).
Research themes with our lab
Resources
Collaborators
Andela Šarić
Buzz Baum
Bassam Hajj
Christophe Leterrier
Florian Jug
Gautam Dey
Guillaume Jacquemet
Joe Grove
Jason Mercer
Loïc Royer
Martin Weigert
Mark Marsh
Simon Foster
Serge Mostowy
Uwe Schmidt
Publications with our group (see more on Google Scholar)
| High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation Romain F Laine, Hannah S Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Tommaso Galgani, Aki Stubb, Gautier Follain, Siân Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques Published in bioRxiv, April 2022 (see preprint) |
| Physical mechanisms of ESCRT-III–driven cell division Lena Harker-Kirschneck, Anne E Hafner, Tina Yao, Andre Arashiro Pulschen, Fredrik Hurtig, Christian Vanhille Campos, Dawid Hryniuk, Sian Culley, Ricardo Henriques, Buzz Baum, Andela Šarić Published in PNAS, January 2022 (see publication) |
| An Introduction to Live-Cell Super-Resolution Imaging Siân Culley, Pedro M Pereira, Romain F Laine, Ricardo Henriques Published in Imaging from Cells to Animals In Vivo, November 2020 (see publication) |
| Closed mitosis requires local disassembly of the nuclear envelope Gautam Dey, Siân Culley, Scott Curran, Ricardo Henriques, Wanda Kukulski, Buzz Baum Published in Nature, August 2020 (see publication or preprint) |
| The proteasome controls ESCRT-III–mediated cell division in an archaeon Gabriel Tarrason Risa, Fredrik Hurtig, Sian Bray, Anne E Hafner, Lena Harker-Kirschneck, Peter Faull, Colin Davis, Dimitra Papatziamou, Delyan R Mutavchiev, Catherine Fan, Leticia Meneguello, Andre Arashiro Pulschen, Gautam Dey, Siân Culley, Mairi Kilkenny, Diorge P Souza, Luca Pellegrini, Robertus AM de Bruin, Ricardo Henriques, Ambrosius P Snijders, Anđela Šarić, Ann-Christin Lindås, Nicholas P Robinson, Buzz Baum Published in Science, August 2020 (see publication or preprint) |
| Live Imaging of a Hyperthermophilic Archaeon Reveals Distinct Roles for Two ESCRT-III Homologs in Ensuring a Robust and Symmetric Division Andre Arashiro Pulschen, Delyan R Mutavchiev, Siân Culley, Kim Nadine Sebastian, Jacques Roubinet, Marc Roubinet, Gabriel Tarrason Risa, Marleen van Wolferen, Chantal Roubinet, Uwe Schmidt, Gautam Dey, Sonja-Verena Albers, Ricardo Henriques, Buzz Baum Published in Current Biology, June 2020 (see publication or preprint) |
| Between life and death: strategies to reduce phototoxicity in super-resolution microscopy Kalina L Tosheva, Yue Yuan, Pedro Matos Pereira, Siân Culley, Ricardo Henriques Published in Journal of Physics D: Applied Physics, January 2020 (see publication) |
| Fix your membrane receptor imaging: Actin cytoskeleton and CD4 membrane organization disruption by chemical fixation Pedro M Pereira, David Albrecht, Siân Culley, Caron Jacobs, Mark Marsh, Jason Mercer, Ricardo Henriques Published in Frontiers in immunology, April 2019 (see publication) |
| Automating multimodal microscopy with NanoJ-Fluidics Pedro Almada, Pedro M Pereira, Siân Culley, Ghislaine Caillol, Fanny Boroni-Rueda, Christina L Dix, Guillaume Charras, Buzz Baum, Romain F Laine, Christophe Leterrier, Ricardo Henriques Published in Nature communications, March 2019 (see publication) |
| NanoJ: a high-performance open-source super-resolution microscopy toolbox Romain F Laine, Kalina L Tosheva, Nils Gustafsson, Robert DM Gray, Pedro Almada, David Albrecht, Gabriel T Risa, Fredrik Hurtig, Ann-Christin Lindås, Buzz Baum, Jason Mercer, Christophe Leterrier, Pedro M Pereira, Siân Culley, Ricardo Henriques Published in Journal of Physics D: Applied Physics, February 2019 (see publication) |
| Content-aware image restoration: pushing the limits of fluorescence microscopy Martin Weigert, Uwe Schmidt, Tobias Boothe, Andreas Müller, Alexandr Dibrov, Akanksha Jain, Benjamin Wilhelm, Deborah Schmidt, Coleman Broaddus, Siân Culley, Mauricio Rocha-Martins, Fabián Segovia-Miranda, Caren Norden, Ricardo Henriques, Marino Zerial, Michele Solimena, Jochen Rink, Pavel Tomancak, Loic Royer, Florian Jug, Eugene W Myers Published in Nature methods, December 2018 (see publication) |
| SRRF: Universal live-cell super-resolution microscopy Siân Culley, Kalina L Tosheva, Pedro Matos Pereira, Ricardo Henriques Published in The international journal of biochemistry & cell biology, August 2018 (see publication) |
| Quantitative mapping and minimization of super-resolution optical imaging artifacts Siân Culley, David Albrecht, Caron Jacobs, Pedro Matos Pereira, Christophe Leterrier, Jason Mercer, Ricardo Henriques Published in Nature methods, April 2018 (see publication) |
| Heterogeneous localisation of membrane proteins in Staphylococcus aureus Felix Weihs, Katarzyna Wacnik, Robert D Turner, Siân Culley, Ricardo Henriques, Simon J Foster Published in Scientific reports, April 2018 (see publication) |
| Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations Nils Gustafsson, Siân Culley, George Ashdown, Dylan M Owen, Pedro Matos Pereira, Ricardo Henriques Published in Nature communications, August 2016 (see publication) |
| Mitochondria mediate septin cage assembly to promote autophagy of Shigella Andrea Sirianni, Sina Krokowski, Damián Lobato‐Márquez, Stephen Buranyi, Julia Pfanzelter, Dieter Galea, Alexandra Willis, Siân Culley, Ricardo Henriques, Gerald Larrouy‐Maumus, Michael Hollinshead, Vanessa Sancho‐Shimizu, Michael Way, Serge Mostowy Published in EMBO reports, July 2016 (see publication) |
| Infection counter: automated quantification of in vitro virus replication by fluorescence microscopy Siân Culley, Greg J Towers, David L Selwood, Ricardo Henriques, Joe Grove Published in Viruses, July 2016 (see publication) |
| PALM and STORM: Into large fields and high-throughput microscopy with sCMOS detectors Pedro Almada, Siân Culley, Ricardo Henriques Published in Methods, October 2015 (see publication) |