Siân Culley


Study of extremophiles and development of optical super-resolution microscopy
Started on January, 2014
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My research centres on developing novel analytical approaches for fluorescence microscopy. I helped develop the Super-Resolution Radial Fluctuations (SRRF) algorithm, which extracts super-resolution information from conventional fluorescence microscopy time-series, and allows for live-cell super-resolution imaging in physiological conditions without specialised fluorophores. I also developed the Super-Resolution Quantitative Image Rating and Reporting of Error Locations (SQUIRREL) algorithm, which is a method for assessing the quality of super-resolution microscopy images. I also develop analytical methods for microbiology, including the creation of image analysis pipelines for characterising nuclear envelope architecture during mitosis in S. pombe (in collaboration with Gautam Dey, LMCB) and characterising cell division in thermophilic Archaea (in collaboration with Uwe Schmidt, MPI-CBG).

Research themes with our lab

Resources

Collaborators

Buzz Baum

Cell Morphogenesis Lab, University College London, UK

Gautam Dey

Cell Morphogenesis Lab, University College London, UK

Joe Grove

Grove Lab, University College London, UK

Jason Mercer

Viral Cell Biology Lab, University of Birmingham, UK

Mark Marsh

Cellular Mechanisms of Virus Entry and Assembly Lab, University College London, UK

Serge Mostowy

Cellular Microbiology Lab, London School of Hygiene and Tropical Medicine, UK

Publications with our group (see more on Google Scholar)

The proteasome controls ESCRT-III–mediated cell division in an archaeon
Gabriel Tarrason Risa, Fredrik Hurtig, Sian Bray, Anne E Hafner, Lena Harker-Kirschneck, Peter Faull, Colin Davis, Dimitra Papatziamou, Delyan R Mutavchiev, Catherine Fan, Leticia Meneguello, Andre Arashiro Pulschen, Gautam Dey, Siân Culley, Mairi Kilkenny, Diorge P Souza, Luca Pellegrini, Robertus AM de Bruin, Ricardo Henriques, Ambrosius P Snijders, Anđela Šarić, Ann-Christin Lindås, Nicholas P Robinson, Buzz Baum
Published in Science, August 2020 (see publication or preprint)
Content-aware image restoration: pushing the limits of fluorescence microscopy
Martin Weigert, Uwe Schmidt, Tobias Boothe, Andreas Müller, Alexandr Dibrov, Akanksha Jain, Benjamin Wilhelm, Deborah Schmidt, Coleman Broaddus, Siân Culley, Mauricio Rocha-Martins, Fabián Segovia-Miranda, Caren Norden, Ricardo Henriques, Marino Zerial, Michele Solimena, Jochen Rink, Pavel Tomancak, Loic Royer, Florian Jug, Eugene W Myers
Published in Nature methods, December 2018 (see publication)