NanoJ-SRRF

SRRF (pronounced as surf) is as a novel open-source and high-performance analytical approach for Live-cell Super-Resolution Microscopy, provided as a fast GPU-enabled ImageJ plugin. SRRF is capable of extracting high-fidelity super-resolution information in modern microscopes (TIRF, widefield and confocals) using conventional fluorophores such as GFP. Compared to other methods, SRRF is capable of live-cell imaging over timescales ranging from minutes to hours, using sample illumination orders of magnitude lower than methods such as PALM, STORM or STED.

🔗Source-Code Repository

Main publication:

Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations *Nils Gustafsson*, *Siân Culley*, George Ashdown, Dylan M Owen, *Pedro Matos Pereira*, *Ricardo Henriques* Published in Nature communications, August 2016 (see publication) Research themes: New Methods, Software Type: Paper, Corresponding author

Other publications using resource:

An Introduction to Live-Cell Super-Resolution Imaging *Siân Culley*, *Pedro M Pereira*, *Romain F Laine*, *Ricardo Henriques* Published in Imaging from Cells to Animals In Vivo, November 2020 (see publication) Research themes: New Methods Type: Book Chapter

Closed mitosis requires local disassembly of the nuclear envelope Gautam Dey, *Siân Culley*, Scott Curran, *Ricardo Henriques*, Wanda Kukulski, Buzz Baum Published in Nature, August 2020 (see publication or preprint) Research themes: Cell Division, Microbiology Type: Paper

The proteasome controls ESCRT-III–mediated cell division in an archaeon Gabriel Tarrason Risa, Fredrik Hurtig, Sian Bray, Anne E Hafner, Lena Harker-Kirschneck, Peter Faull, Colin Davis, Dimitra Papatziamou, Delyan R Mutavchiev, Catherine Fan, Leticia Meneguello, Andre Arashiro Pulschen, Gautam Dey, *Siân Culley*, Mairi Kilkenny, Diorge P Souza, Luca Pellegrini, Robertus AM de Bruin, *Ricardo Henriques*, Ambrosius P Snijders, Anđela Šarić, Ann-Christin Lindås, Nicholas P Robinson, Buzz Baum Published in Science, August 2020 (see publication or preprint) Research themes: Cell Division Type: Paper

Fluctuation-Based Super-Resolution Traction Force Microscopy Aki Stubb, *Romain F Laine*, Camilo Guzmán, *Ricardo Henriques*, Guillaume Jacquemet, Johanna Ivaska Published in Nano Letters, March 2020 (see publication) Research themes: New Methods, Software Type: Paper

Nuclear pores as versatile reference standards for quantitative superresolution microscopy Jervis Vermal Thevathasan, Maurice Kahnwald, Konstanty Cieśliński, Philipp Hoess, Sudheer Kumar Peneti, Manuel Reitberger, Daniel Heid, Krishna Chaitanya Kasuba, Sarah Janice Hoerner, Yiming Li, Yu-Le Wu, Markus Mund, Ulf Matti, *Pedro Matos Pereira*, *Ricardo Henriques*, Bianca Nijmeijer, Moritz Kueblbeck, Vilma Jimenez Sabinina, Jan Ellenberg, Jonas Ries Published in Nature methods, October 2019 (see publication) Research themes: Structural Biology, New Methods Type: Paper

Fix your membrane receptor imaging: Actin cytoskeleton and CD4 membrane organization disruption by chemical fixation *Pedro M Pereira*, *David Albrecht*, *Siân Culley*, *Caron Jacobs*, Mark Marsh, Jason Mercer, *Ricardo Henriques* Published in Frontiers in immunology, April 2019 (see publication) Research themes: Cell Signalling, New Methods Type: Paper, Corresponding author

Automating multimodal microscopy with NanoJ-Fluidics *Pedro Almada*, *Pedro M Pereira*, *Siân Culley*, Ghislaine Caillol, Fanny Boroni-Rueda, Christina L Dix, Guillaume Charras, Buzz Baum, *Romain F Laine*, Christophe Leterrier, *Ricardo Henriques* Published in Nature communications, March 2019 (see publication) Research themes: New Methods, Hardware Type: Paper, Corresponding author

Real time multi-modal super-resolution microscopy through Super-Resolution Radial Fluctuations (SRRF-Stream) Justin Cooper, Mark Browne, Hugh Gribben, Martin Catney, Colin Coates, Alan Mullan, Geraint Wilde, *Ricardo Henriques* Published in SPIE, February 2019 (see publication) Research themes: New Methods Type: Conference Paper, Corresponding author

NanoJ: a high-performance open-source super-resolution microscopy toolbox *Romain F Laine*, *Kalina L Tosheva*, *Nils Gustafsson*, *Robert DM Gray*, *Pedro Almada*, *David Albrecht*, Gabriel T Risa, Fredrik Hurtig, Ann-Christin Lindås, Buzz Baum, Jason Mercer, Christophe Leterrier, *Pedro M Pereira*, *Siân Culley*, *Ricardo Henriques* Published in Journal of Physics D: Applied Physics, February 2019 (see publication) Research themes: New Methods, Software Type: Paper, Corresponding author

Content-aware image restoration: pushing the limits of fluorescence microscopy Martin Weigert, Uwe Schmidt, Tobias Boothe, Andreas Müller, Alexandr Dibrov, Akanksha Jain, Benjamin Wilhelm, Deborah Schmidt, Coleman Broaddus, *Siân Culley*, Mauricio Rocha-Martins, Fabián Segovia-Miranda, Caren Norden, *Ricardo Henriques*, Marino Zerial, Michele Solimena, Jochen Rink, Pavel Tomancak, Loic Royer, Florian Jug, Eugene W Myers Published in Nature methods, December 2018 (see publication) Research themes: New Methods, Software Type: Paper

SRRF: Universal live-cell super-resolution microscopy *Siân Culley*, *Kalina L Tosheva*, *Pedro Matos Pereira*, *Ricardo Henriques* Published in The international journal of biochemistry & cell biology, August 2018 (see publication) Research themes: New Methods, Software Type: Paper, Corresponding author

Heterogeneous localisation of membrane proteins in Staphylococcus aureus Felix Weihs, Katarzyna Wacnik, Robert D Turner, *Siân Culley*, *Ricardo Henriques*, Simon J Foster Published in Scientific reports, April 2018 (see publication) Research themes: Microbiology Type: Paper

Mitochondria mediate septin cage assembly to promote autophagy of Shigella Andrea Sirianni, Sina Krokowski, Damián Lobato‐Márquez, Stephen Buranyi, Julia Pfanzelter, Dieter Galea, Alexandra Willis, *Siân Culley*, *Ricardo Henriques*, Gerald Larrouy‐Maumus, Michael Hollinshead, Vanessa Sancho‐Shimizu, Michael Way, Serge Mostowy Published in EMBO reports, July 2016 (see publication) Research themes: Host-Pathogen Interactions Type: Paper