Paired image-to-image translation of 2D images. pix2pix is a deep-learning method that can be used to translate one type of images into another. While pix2pix can potentially be used for any type of image-to-image translation, we demonstrate that it can be used to predict a fluorescent image from another fluorescent image. Note - visit the ZeroCostDL4Mic wiki to check the original publications this network is based on and make sure you cite these.